SMLocalizer, a GPU accelerated ImageJ plugin for single molecule localization microscopy
نویسندگان
چکیده
منابع مشابه
SMLocalizer, a GPU accelerated ImageJ plugin for single molecule localization microscopy
Summary SMLocalizer combines the availability of ImageJ with the power of GPU processing for fast and accurate analysis of single molecule localization microscopy data. Analysis of 2D and 3D data in multiple channels is supported. Availability and implementation Plugin freely available for Fiji and ImageJ2.0 through https://sourceforge.net/projects/smlocalizer/. Plugin also available for cont...
متن کاملPractical considerations for single molecule localization microscopy
This is an especially exciting era in biological optical microscopy, a series of new and accessible far-field ‘superresolution’ fluorescence techniques are changing the way we view cells. These techniques surpass the traditional resolution limit dictated by the diffraction of light, approaching levels previously occupied solely by electron microscopy. Herein the authors review and discuss some ...
متن کاملWavelet analysis for single molecule localization microscopy.
Localization of single molecules in microscopy images is a key step in quantitative single particle data analysis. Among them, single molecule based super-resolution optical microscopy techniques require high localization accuracy as well as computation of large data sets in the order of 10(5) single molecule detections to reconstruct a single image. We hereby present an algorithm based on imag...
متن کاملChallenges in quantitative single molecule localization microscopy.
Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. It has already been used for this purpose in varied fields in biology, ranging from molecular biology to neuroscience. In this review articl...
متن کاملLocalization accuracy in single-molecule microscopy.
One of the most basic questions in single-molecule microscopy concerns the accuracy with which the location of a single molecule can be determined. Using the Fisher information matrix it is shown that the limit of the localization accuracy for a single molecule is given by, lambda(em)/2pi n(a) square root of gammaAt, where lambda(em), n(a), gamma, A, and t denote the emission wavelength of the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioinformatics
سال: 2017
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/btx553